NIH, Wuhan were working on monkeypox

Wuhan Institute of Virology published on monkeypox research 3 months ago, NIH also have been researching monkeypox treatment

Total funding, $9,824,009

The funding supports a clinical trial to identify effective treatments for monkeypox

A re-emerging pathogen

A disease of epidemic potential

Causes significant morbidity and can result in death

Human cases have been increasing in sub-Saharan Africa since 2000

Sporadic outbreaks outside of Africa have occurred

Similarity between MPXV and the variola virus,

coupled with concerns about the potential of the variola virus as a potential bioterrorism agent,

have placed monkeypox treatments at the forefront of public health and scientific research agendas in many countries.

Article in Virologica Sinica?

Efficient assembly of a large fragment of monkeypox virus genome as a qPCR template using dual-selection based transformation-associated recombination (Feb 2022)

Since MPXV infection has never been associated with an outbreak in China, the viral genomic material required for qPCR detection is unavailable.

Using viral DNA recombinations

Transformation-associated recombination (TAR),

to assemble large DNA constructs

A 55-kb genomic fragment of monkeypox virus in VL6-48B (yeast cells)

What is Virologica Sinica?

Virologica Sinica, the official journal of Chinese Society for Microbiology,

will serve as a platform for the communication and exchange of academic information and ideas in an international context.

Discussion section

However, this DNA assembly tool applied in virological research could also raise potential security concerns,

especially when the assembled product contains a full set of genetic material that can be recovered into a contagious pathogen.

Recently, a group of scientists was funded by a biotech company to synthesize a full-length horsepox virus genome and recover it into an infectious virus (Noyce et al., 2018).

Not surprisingly, such a controversial achievement has received enormous attention and raised global debate on its biosecurity implications (DiEuliis et al., 2017; Koblentz, 2017, 2018; DiEuliis and Gronvall, 2018).

In this study, although a full-length viral genome would be the ideal reference template for detecting MPXV by qPCR, we only sought to assemble a 55-kb viral fragment, less than one-third of the MPXV genome.

This assembly product is fail-safe by virtually eliminating any risk of recovering into an infectious virus while providing multiple qPCR targets for detecting MPXV or other Orthopoxviruses (Li et al., 2010).

Our world in data monkeypox

Spain and Belgium ‘gay events’


Cases, + 11 = 190

20,000 doses of a smallpox vaccine

offered to close contacts of those diagnosed with monkeypox

reduce the risk of symptomatic infection and severe illness

Dr Rosamund Lewis, WHO

It’s very important to describe this because it appears to be an increase in a mode of transmission that may have been under-recognised in the past

unfortunate if monkeypox exploit the immunity gap left by smallpox 40 years ago

there is still a window to close the outbreak

Written by Dr. John Campbell

Hello Everyone,My name is John Campbell and I am a retired Nurse Teacher and A and E nurse based in England. I also do some teaching in Asia and Africa when time permits. These videos are to help students to learn the background to all forms of health care. My PhD focused on the development of open learning resources for nurses nationally and internationally.


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  1. "Routine vaccination of the American public against smallpox stopped in 1972 after the disease was eradicated in the United States." This is the replacement niche, a now mostly virus naive population.

  2. You’re really growing on me Dr. John. In the early days, I found it hard to tell if you were sceptical at all. Now, that you are fully prepared to “respectfully” question the dogma, I’m all ears. Thanks.

  3. Sounds like Europeans are reaping what their forefathers the "conquistadors" sowed
    Cortez spread disease all over south America so he could steal their gold and silver etc .
    There is nothing new under the sun. Ppl have been spreading disease amongst their enemies for centuries.

  4. Just been to local safari park, and there is monkeys and gibbons, and other animals everywhere. Which is surprising! To my surprise I managed to grab a word with a monkey on my wing mirror whilst playing with his lipstick 🤨 and he said, there is a virus that is infecting all animals in the park! Called human twatitus, and he told me 95 percent of humans are twats. I told him, humans are clones of each other that eat each other’s views and believe it, he offered me a tree to play in, I said go fuck yourselves I don’t want monkey poxs 😂😂😂😂

  5. As much as we fear this type of stories. We have to ask yourselves, how do you formulate a test or treatment for a virus, even bacteria if we don't look into its DNA? Furthermore, If we wanted to do thousands of reproducible tests, I am sure 500 human samples is not enough, adding to it the logistics and biohazards of transporting such dangerous specimens intercontinental.

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